DOI Seite / Zitierlink:
https://doi.org/10.11588/diglit.48120#0027
Abstracts
9
lished sequences of the snRNP A and C proteins [2, 3], spanning six up to eight
amino acids, were found. No homology with the D protein as published by
Rokeach et al. [4] was observed.
If compared with recently published cDNA sequences coding for a B/B' prote-
in and for the related N-protein [5], our B clone was highly related to the B/B'
clone as described. However, an additional guanidine residue in our clone lead to
the introduction of a termination codon after amino acid 231, therefore shorten-
ing the protein by 56 amino acids at the C-terminal end. Furthermore, three extra
amino acids were present in our clone at positions 172, 201 and 217, and amino
acid substitutions were found at positions 5, 6, 173 and 218. Both B' and N con-
tained 240 amino acids, with an homology of 92.5 % at the amino acid level. Since
the non-coding regions of the two cDNAs are completely different, these proteins
must originate from different genes.
References
1. Tan, E. M. (1989): Antinuclear antibodies: Diagnostic markers for autoimmune dis-
eases and probes for cell biology. Adv. Immunol. 44, 93
2. Sillekens, P. T.G., Habets, W. J., Beijer, R. P., Van Venrooij, W. J. (1986): cDNA
cloning of the human U1 snRNA-associated A protein: extensive homology between
U1 and U2 snRNP-specific proteins. EMBO J. 6, 3841
3. Sillekens, P.T.G., Beijer, R.P., Habets, W. J., Van Venrooij, W. (1988): Human
U1 snRNP-specific C protein: Complete cDNA and protein sequence and identifica-
tion of a multigene family in mammals. Nucleic. Acids Res. 16, 8307
4. Rokeach, L.A., Haselby, J. A., Hoch, S.O. (1988): Molecular cloning of a cDNA
encoding the human Sm-D autoantigen. Proc. Natl. Acad. Sci. USA 85, 4832
5. Schmauss, C., McAllister, G., Ohosone, Y, Hardin, J. A., Lerner, M.R. (1989):
A comparison of snRNP-associated Sm-autoantigens: human N, rat N and human
B/B'. Nucleic Acids Res. 17, 1733
Antibodies to Ribonucleoprotein (RNP) from Autoimmune Sera
Detected by Immunoblot on Tissue Extracts
E. Dayer, J.-M. Dayer, J.-P. Despont, and A. Cruchaud
Div. of Immunology and Allergy, Dept, of Medicine, University Hospital, 1211 Geneva 4,
Switzerland
To establish the protein specificities associated with Sm, U1 RNP, Ro and La
antigenicity, 19 counterimmunoelectrophosis (CIE)-positive sera from patients
9
lished sequences of the snRNP A and C proteins [2, 3], spanning six up to eight
amino acids, were found. No homology with the D protein as published by
Rokeach et al. [4] was observed.
If compared with recently published cDNA sequences coding for a B/B' prote-
in and for the related N-protein [5], our B clone was highly related to the B/B'
clone as described. However, an additional guanidine residue in our clone lead to
the introduction of a termination codon after amino acid 231, therefore shorten-
ing the protein by 56 amino acids at the C-terminal end. Furthermore, three extra
amino acids were present in our clone at positions 172, 201 and 217, and amino
acid substitutions were found at positions 5, 6, 173 and 218. Both B' and N con-
tained 240 amino acids, with an homology of 92.5 % at the amino acid level. Since
the non-coding regions of the two cDNAs are completely different, these proteins
must originate from different genes.
References
1. Tan, E. M. (1989): Antinuclear antibodies: Diagnostic markers for autoimmune dis-
eases and probes for cell biology. Adv. Immunol. 44, 93
2. Sillekens, P. T.G., Habets, W. J., Beijer, R. P., Van Venrooij, W. J. (1986): cDNA
cloning of the human U1 snRNA-associated A protein: extensive homology between
U1 and U2 snRNP-specific proteins. EMBO J. 6, 3841
3. Sillekens, P.T.G., Beijer, R.P., Habets, W. J., Van Venrooij, W. (1988): Human
U1 snRNP-specific C protein: Complete cDNA and protein sequence and identifica-
tion of a multigene family in mammals. Nucleic. Acids Res. 16, 8307
4. Rokeach, L.A., Haselby, J. A., Hoch, S.O. (1988): Molecular cloning of a cDNA
encoding the human Sm-D autoantigen. Proc. Natl. Acad. Sci. USA 85, 4832
5. Schmauss, C., McAllister, G., Ohosone, Y, Hardin, J. A., Lerner, M.R. (1989):
A comparison of snRNP-associated Sm-autoantigens: human N, rat N and human
B/B'. Nucleic Acids Res. 17, 1733
Antibodies to Ribonucleoprotein (RNP) from Autoimmune Sera
Detected by Immunoblot on Tissue Extracts
E. Dayer, J.-M. Dayer, J.-P. Despont, and A. Cruchaud
Div. of Immunology and Allergy, Dept, of Medicine, University Hospital, 1211 Geneva 4,
Switzerland
To establish the protein specificities associated with Sm, U1 RNP, Ro and La
antigenicity, 19 counterimmunoelectrophosis (CIE)-positive sera from patients