DOI Seite / Zitierlink:
https://doi.org/10.11588/diglit.48120#0037
Abstracts
19
Chronic Graft-versus-Host Disease. A Model for the Study of the
Autoimmune Phenomenon
C. Gelpi, Ma. A. Martinez, S. Vidal, A. Alguero, J. A. Hardin,
and J. L. Rodriguez-Sanchez
Hospital de la Santa Crenu i Sant Pau, Hospital Universitari de la Facultat de Medicina
de la Universitat Autonoma de Barcelona, San Antonio Ma. Claret, 167, 08025 Barcelona,
Spain
Chronic graft-versus-host disease provides an experimental model for the
study of the autoimmune phenomenon. In this paper we describe the clinical
characteristics and serological aspects of Fl hybrid mice (Balb/cxA/J) to which
spleen and lymph node cells of one or the other of the parental strains were in-
travenously injected.
We found that A/J cells induced the development of immunocomplex
glomerulonephritis in 55 °7o of the animals, and 25% developed inflamation of the
soft parts of the upper extremities. All of the mice of this model had antinuclear
antibodies, mainly antibodies to UlsnRNP and anti-U3snRNP (50% and 30%
respectively). Anti-DNA antibodies and anti-histone antibodies were present in
90% and 15% of the mice. The data of this study showed that this model had
clinical and serological aspects of MCTD with scleroderma and SLE, with elec-
tromyographic aspects of myositis.
In contrast, Balb/c cells induced the development of vasculitis (55%);
alopecia (40%); and less frequently, renal involvement. Some (50%) of the
animals of this group later showed inflamation of the joints of the paws. All of
the mice of this model had antinuclear antibodies. Anti-dsDNA, anti-histones
and anti-snRNP antibodies were present in low titers in 50%, 25% and 35% of
the animals respectively. Rheumatoid factors were the most prominent finding in
the animals of this model (69%), as well as their correlation with synovitis. We
conclude that this model of GVH disease shows aspects of rheumatoid arthritis
and SLE.
We also describe the clinical and serological aspects induced in Fl hybrid mice
(C57BL/10X DBA/2) after the intravenous injection of lymphoid cells from
DBA/2 strain. In this model the prominent finding was the development of a
renal disease in 65% of the animals. The antigenic specificities recognized by the
antinuclear antibodies of these animals included anti-dsDNA (80%), anti-Sm
(30%), anti-rRNA (70%), anti-tRNA (85%) and anti-histones (45%). Anti-
histone antibodies recognized the epitopes located at the same fragments of the
antigen that include the epitopes reactive with the human SLE sera. We conclude
that this GVHD model had clinical and serological characteristics of active SLE,
and anti-tRNA antibodies. These antibodies are frequent in human patients with
polimyositis.
19
Chronic Graft-versus-Host Disease. A Model for the Study of the
Autoimmune Phenomenon
C. Gelpi, Ma. A. Martinez, S. Vidal, A. Alguero, J. A. Hardin,
and J. L. Rodriguez-Sanchez
Hospital de la Santa Crenu i Sant Pau, Hospital Universitari de la Facultat de Medicina
de la Universitat Autonoma de Barcelona, San Antonio Ma. Claret, 167, 08025 Barcelona,
Spain
Chronic graft-versus-host disease provides an experimental model for the
study of the autoimmune phenomenon. In this paper we describe the clinical
characteristics and serological aspects of Fl hybrid mice (Balb/cxA/J) to which
spleen and lymph node cells of one or the other of the parental strains were in-
travenously injected.
We found that A/J cells induced the development of immunocomplex
glomerulonephritis in 55 °7o of the animals, and 25% developed inflamation of the
soft parts of the upper extremities. All of the mice of this model had antinuclear
antibodies, mainly antibodies to UlsnRNP and anti-U3snRNP (50% and 30%
respectively). Anti-DNA antibodies and anti-histone antibodies were present in
90% and 15% of the mice. The data of this study showed that this model had
clinical and serological aspects of MCTD with scleroderma and SLE, with elec-
tromyographic aspects of myositis.
In contrast, Balb/c cells induced the development of vasculitis (55%);
alopecia (40%); and less frequently, renal involvement. Some (50%) of the
animals of this group later showed inflamation of the joints of the paws. All of
the mice of this model had antinuclear antibodies. Anti-dsDNA, anti-histones
and anti-snRNP antibodies were present in low titers in 50%, 25% and 35% of
the animals respectively. Rheumatoid factors were the most prominent finding in
the animals of this model (69%), as well as their correlation with synovitis. We
conclude that this model of GVH disease shows aspects of rheumatoid arthritis
and SLE.
We also describe the clinical and serological aspects induced in Fl hybrid mice
(C57BL/10X DBA/2) after the intravenous injection of lymphoid cells from
DBA/2 strain. In this model the prominent finding was the development of a
renal disease in 65% of the animals. The antigenic specificities recognized by the
antinuclear antibodies of these animals included anti-dsDNA (80%), anti-Sm
(30%), anti-rRNA (70%), anti-tRNA (85%) and anti-histones (45%). Anti-
histone antibodies recognized the epitopes located at the same fragments of the
antigen that include the epitopes reactive with the human SLE sera. We conclude
that this GVHD model had clinical and serological characteristics of active SLE,
and anti-tRNA antibodies. These antibodies are frequent in human patients with
polimyositis.