DOI Page / Citation link:
https://doi.org/10.11588/diglit.48120#0046
28
Molecular and Cell Biology of Autoantibodies and Autoimmunity
tionated on urea-PAGE and detected by autoradiography. The results of our study
are summarized in the table.
Table: Frequencies of autoantibodies to ribonucleoproteins
Antibodies to: (precipitated RNA)
SLE
(126)
(%)
PSS
(113)
W
PM/DM
(51)
(%)
Overlap
(115)
(%)
U1RNP
(U1RNA)
21
12
4
46
Ro/SS-A
(Y1-Y5RNA)
42
20
10
34
La/SS-B
(La-RNA)
17
7
2
10
Sm
(Ul, U2, U4-sRNA)
29
4
0
29
Ribosome
(rRNA)
4
1
2
1
U3RNP
(U3RNA)
1
7
0
1
7-2RNP
(7-2RNA)
1
4
0
1
Jo-1
(tRNAhls)
0
0
29
3
PL-7
(tRNAthr)
0
0
4
1
PL-12
(tRNAala)
0
0
2
0
SRP
(7SL-RNA)
0
0
6
0
U1/U2R-
NP
(U1/U2RNA)
0
0
0
9
Anti-U3RNP and anti-7-2RNP antibodies defined among sera containing an-
ti-nucleolar reactivity in immunofluorescence appeared to be specific markers for
PSS. Anti-U3RNP antibodies were associated with PSS characterized by a low
prevalence of lung involvement. Anti-7-2RNP antibodies were detected in patients
with limited form of scleroderma. Antibodies to aminoacyl-tRNA synthetases
(Jo-1, PL-7 and PL-12) were strongly associated with PM/DM with chronic in-
terstitial lung disease characterized by “shrinking lung” and polyarthritis. Anti-
SRP (Signal Recognition Particle) antibodies were detected specifically in patients
with intractable PM/DM. Antibodies which precipitated selectively U1 and
U2RNa (anti-Ul/U2RNP) were identified in sera with anti-UIRNP only by im-
munodiffuison, and strongly associated with overlap syndrome characterized by
scleroderma and inflammatory myositis.
Detailed analysis of the fine specificities of autoantibodies reactive with RNPs
disclosed the presence of unique patient groups which were not identified by the
conventional immunological methods.
Molecular and Cell Biology of Autoantibodies and Autoimmunity
tionated on urea-PAGE and detected by autoradiography. The results of our study
are summarized in the table.
Table: Frequencies of autoantibodies to ribonucleoproteins
Antibodies to: (precipitated RNA)
SLE
(126)
(%)
PSS
(113)
W
PM/DM
(51)
(%)
Overlap
(115)
(%)
U1RNP
(U1RNA)
21
12
4
46
Ro/SS-A
(Y1-Y5RNA)
42
20
10
34
La/SS-B
(La-RNA)
17
7
2
10
Sm
(Ul, U2, U4-sRNA)
29
4
0
29
Ribosome
(rRNA)
4
1
2
1
U3RNP
(U3RNA)
1
7
0
1
7-2RNP
(7-2RNA)
1
4
0
1
Jo-1
(tRNAhls)
0
0
29
3
PL-7
(tRNAthr)
0
0
4
1
PL-12
(tRNAala)
0
0
2
0
SRP
(7SL-RNA)
0
0
6
0
U1/U2R-
NP
(U1/U2RNA)
0
0
0
9
Anti-U3RNP and anti-7-2RNP antibodies defined among sera containing an-
ti-nucleolar reactivity in immunofluorescence appeared to be specific markers for
PSS. Anti-U3RNP antibodies were associated with PSS characterized by a low
prevalence of lung involvement. Anti-7-2RNP antibodies were detected in patients
with limited form of scleroderma. Antibodies to aminoacyl-tRNA synthetases
(Jo-1, PL-7 and PL-12) were strongly associated with PM/DM with chronic in-
terstitial lung disease characterized by “shrinking lung” and polyarthritis. Anti-
SRP (Signal Recognition Particle) antibodies were detected specifically in patients
with intractable PM/DM. Antibodies which precipitated selectively U1 and
U2RNa (anti-Ul/U2RNP) were identified in sera with anti-UIRNP only by im-
munodiffuison, and strongly associated with overlap syndrome characterized by
scleroderma and inflammatory myositis.
Detailed analysis of the fine specificities of autoantibodies reactive with RNPs
disclosed the presence of unique patient groups which were not identified by the
conventional immunological methods.