DOI Page / Citation link:
https://doi.org/10.11588/diglit.48120#0057
Abstracts
39
strated in 34 of 35 ACA positive sera by immunoblotting using the crude antigen.
All of the 34 sera reacted with 17 kD protein purified by the immunoaffinity chro-
matography. Two patients with SR had both ACA and anti-Ul-RNP antibody.
These sera reacted with 68 kD, A and 17 kD protein. One patient with scleroder-
ma had ACA and anti-Scl-70 antibody.
These studies showed that the predominant ANA in scleroderma was anti-
Scl-70 antibody and the main ANA in SR were ACA and anti-Ul-RNP an-
tibodies. Furthermore, immunoblotting using crude antigens and purified 17 kD
centomeric antigen was useful for detection of two different system antibodies in-
cluding ACA and anti-ENA antibody.
Desmin Antibodies in Acute Myopericarditis
P. Kurki, J. Karjalainen, A. Hautanen, and I. Virtanen
Dept, of Bacteriology and Immunology, Dept, of Anatomy, University of Helsinki, First
Dept, of Medicine, Helsinki University Central Hospital, and Central Military Hospital I,
Helsinki, Finland
The role of autoantibodies against cardiac tissue in the pathogenesis of acute
(infectious) myopericarditis is poorly understood - partly because the specificity
of the antibodies is unknown (Woodroff, 1980). We assay circulating an-
tibodies against desmin, the main component of intermediate filaments of muscle
cells (Lazarides, 1982), in 18 patients with acute infectious myopericarditis
(AM), in ten patients with acute myocardial infarction, in 24 patients with acute
uncomplicated infections, and in 68 blood donors by an enzyme-linked im-
munosorbent assay using purified desmin from Purkinje fibers of cow heart as
antigen. In addition, anti-heart antibodies were assayed by indirect immunofluo-
rescence using rat heart as the target.
Anti-heart antibodies were detected in all patient groups: Antibodies reacting
with arterial smooth muscle were detected in 67% of patients with acute myocar-
ditis (AM), 70% of patients with myocardial infarction (MI), and in 16% of pa-
tients with acute uncomplicated infections (Al). Antibodies giving a striational
staining pattern of muscle fibers were seen in 39%, 10%, and 48% of patients
with AM, MI, and Al, respectively.
The cut off point for the anti-desmin assay were set at the mean+ 2 SD of the
blood donors values. The results are seen in Table 1.
39
strated in 34 of 35 ACA positive sera by immunoblotting using the crude antigen.
All of the 34 sera reacted with 17 kD protein purified by the immunoaffinity chro-
matography. Two patients with SR had both ACA and anti-Ul-RNP antibody.
These sera reacted with 68 kD, A and 17 kD protein. One patient with scleroder-
ma had ACA and anti-Scl-70 antibody.
These studies showed that the predominant ANA in scleroderma was anti-
Scl-70 antibody and the main ANA in SR were ACA and anti-Ul-RNP an-
tibodies. Furthermore, immunoblotting using crude antigens and purified 17 kD
centomeric antigen was useful for detection of two different system antibodies in-
cluding ACA and anti-ENA antibody.
Desmin Antibodies in Acute Myopericarditis
P. Kurki, J. Karjalainen, A. Hautanen, and I. Virtanen
Dept, of Bacteriology and Immunology, Dept, of Anatomy, University of Helsinki, First
Dept, of Medicine, Helsinki University Central Hospital, and Central Military Hospital I,
Helsinki, Finland
The role of autoantibodies against cardiac tissue in the pathogenesis of acute
(infectious) myopericarditis is poorly understood - partly because the specificity
of the antibodies is unknown (Woodroff, 1980). We assay circulating an-
tibodies against desmin, the main component of intermediate filaments of muscle
cells (Lazarides, 1982), in 18 patients with acute infectious myopericarditis
(AM), in ten patients with acute myocardial infarction, in 24 patients with acute
uncomplicated infections, and in 68 blood donors by an enzyme-linked im-
munosorbent assay using purified desmin from Purkinje fibers of cow heart as
antigen. In addition, anti-heart antibodies were assayed by indirect immunofluo-
rescence using rat heart as the target.
Anti-heart antibodies were detected in all patient groups: Antibodies reacting
with arterial smooth muscle were detected in 67% of patients with acute myocar-
ditis (AM), 70% of patients with myocardial infarction (MI), and in 16% of pa-
tients with acute uncomplicated infections (Al). Antibodies giving a striational
staining pattern of muscle fibers were seen in 39%, 10%, and 48% of patients
with AM, MI, and Al, respectively.
The cut off point for the anti-desmin assay were set at the mean+ 2 SD of the
blood donors values. The results are seen in Table 1.