DOI Page / Citation link:
https://doi.org/10.11588/diglit.48120#0077
Abstracts
59
Clinical Significance of Anti-Ubiquitin Antibodies in Systemic Lupus
Erythematosus
S. Muller, P. Joubaud, J. P. Briand, S. Plaue, and M. H.V. Van Regenmortel
Laboratoire d’lmmunochimie, Institut de Biologie Moleculaire et Cellulaire, CNRS,
Strasbourg, France
Antibodies to ubiquitin and to ubiquitinated histones have been found in the
sera of patients with systemic lupus erythematosus (SLE) using an enzyme-linked
immunosorbent assay (ELISA) and immunoblotting experiments [1]. In ELISA,
the reaction could be measured with ubiquitin and with synthetic peptides cor-
responding either to residues 22-45 of the ubiquitin molecule or to the branched
moiety of ubiquitinated histone H2A (T peptides).
The pathogenic and clinical significance of antibodies to ubiquitin in SLE re-
mains unclear. In an attempt to correlate the presence of these autoantibodies
with the clinical pattern of disease, the appearance of IgG antibodies reacting
with whole ubiquitin and synthetic fragments was measured in SLE patients at
different stages of the disease. The results were compared to those obtained by
measuring the reactivity of sera to usual SLE markers such as native double-
stranded DNA and Sm. Our findings indicate that the presence of antibodies to
ubiquitin correlates with specific clinical phases of the disease and that they could
represent an early marker for SLE.
Reference
1. Muller, S., Briand, J.P., Van Regenmortel, M.H.V. (1988): Presence of an-
tibodies to ubiquitin during the autoimmune response associated with systemic lupus
erythematosus. Proc. Natl. Acad. Sci. USA 85, 8176-8180
The Natural History of PCNA/Cyclin
P. K. Nakane
Department of Anatomy, Nagasaki University School of Medicine, Nagasaki, Japan
PCNA/cyclin was originally described in proliferating mammalian cells as a
nuclear protein with an apparent molecular weight of 33 000-36000 and was
59
Clinical Significance of Anti-Ubiquitin Antibodies in Systemic Lupus
Erythematosus
S. Muller, P. Joubaud, J. P. Briand, S. Plaue, and M. H.V. Van Regenmortel
Laboratoire d’lmmunochimie, Institut de Biologie Moleculaire et Cellulaire, CNRS,
Strasbourg, France
Antibodies to ubiquitin and to ubiquitinated histones have been found in the
sera of patients with systemic lupus erythematosus (SLE) using an enzyme-linked
immunosorbent assay (ELISA) and immunoblotting experiments [1]. In ELISA,
the reaction could be measured with ubiquitin and with synthetic peptides cor-
responding either to residues 22-45 of the ubiquitin molecule or to the branched
moiety of ubiquitinated histone H2A (T peptides).
The pathogenic and clinical significance of antibodies to ubiquitin in SLE re-
mains unclear. In an attempt to correlate the presence of these autoantibodies
with the clinical pattern of disease, the appearance of IgG antibodies reacting
with whole ubiquitin and synthetic fragments was measured in SLE patients at
different stages of the disease. The results were compared to those obtained by
measuring the reactivity of sera to usual SLE markers such as native double-
stranded DNA and Sm. Our findings indicate that the presence of antibodies to
ubiquitin correlates with specific clinical phases of the disease and that they could
represent an early marker for SLE.
Reference
1. Muller, S., Briand, J.P., Van Regenmortel, M.H.V. (1988): Presence of an-
tibodies to ubiquitin during the autoimmune response associated with systemic lupus
erythematosus. Proc. Natl. Acad. Sci. USA 85, 8176-8180
The Natural History of PCNA/Cyclin
P. K. Nakane
Department of Anatomy, Nagasaki University School of Medicine, Nagasaki, Japan
PCNA/cyclin was originally described in proliferating mammalian cells as a
nuclear protein with an apparent molecular weight of 33 000-36000 and was