DOI Seite / Zitierlink:
https://doi.org/10.11588/diglit.48120#0081
Abstracts
63
sistent with the idea that the B polypeptides associate with U small RNAs in-
directly through interaction with other polypeptides. Except for a 50 amino acid
insert near its carboxy terminus, the cDNAB sequence is nearly homologous at
the aminoacid level with polypeptide N, a newly recognized tissue specific compo-
nent of Sm snRNPs. Structural differences in B and N polypeptides may regulate
tissue specific alternative splicing of mRNA. Several studies have been directed
at understanding the relationship of the two B polypeptides. mRNAs which
hybridize with cDNAS3~4 are translated in vitro into both polypeptides. In con-
trast, in vitro expression of the full length cDNAB yields only the B polypeptide.
These results suggest that there are two related, but independent mRNAs for
B7B.
Studies of individual autoantigenic epitopes demonstrate that anti-Sm an-
tibodies eluted from the cDNAS3-4 fusion protein immunoprecipitate only the
U1 snRNP in some cases and all Sm snRNPs in others. This observation is consis-
tent with the idea that cDNAS3-4 encodes two regions of the B polypeptide: one
that is accessible to antibodies on the U1 snRNP alone and another that is avail-
able for interaction on all of the Sm snRNPs.
The Induction of Anti-DNA Antibodies in Normal Mice by
Immunization with Bacterial DNA
D. S. Pisetsky, J. P. Grudier, and G. S. Gilkeson
Division of Rheumatology and Immunology, Duke University Medical Center, Durham,
NC 27705, USA
To investigate the role of DNA in the induction of anti-DNA antibodies in
systemic lupus erythematosus (SLE), the immune response of mice to bacterial
DNA was investigated. BALB/c mice immunized with E. coli (EC) single-stranded
DNA (ssDNA) in complexes with mBSA produced antibody levels much greater
than similar immunization with calf thymus (CT) DNA. The induced antibodies
contained a population specific for EC DNA as well as a population crossreactive
with CT as well as other DNAs. The induced antibodies bound moreover to a
variety of synthetic polynucleotides including poly dC, poly I, and poly di
without reactivity to poly dT or poly dU. In contrast, immunization with double-
stranded (ds) E. coli DNA elicited antibodies specific for ds E. coli DNA without
crossreactivity to mammalian DNA.
63
sistent with the idea that the B polypeptides associate with U small RNAs in-
directly through interaction with other polypeptides. Except for a 50 amino acid
insert near its carboxy terminus, the cDNAB sequence is nearly homologous at
the aminoacid level with polypeptide N, a newly recognized tissue specific compo-
nent of Sm snRNPs. Structural differences in B and N polypeptides may regulate
tissue specific alternative splicing of mRNA. Several studies have been directed
at understanding the relationship of the two B polypeptides. mRNAs which
hybridize with cDNAS3~4 are translated in vitro into both polypeptides. In con-
trast, in vitro expression of the full length cDNAB yields only the B polypeptide.
These results suggest that there are two related, but independent mRNAs for
B7B.
Studies of individual autoantigenic epitopes demonstrate that anti-Sm an-
tibodies eluted from the cDNAS3-4 fusion protein immunoprecipitate only the
U1 snRNP in some cases and all Sm snRNPs in others. This observation is consis-
tent with the idea that cDNAS3-4 encodes two regions of the B polypeptide: one
that is accessible to antibodies on the U1 snRNP alone and another that is avail-
able for interaction on all of the Sm snRNPs.
The Induction of Anti-DNA Antibodies in Normal Mice by
Immunization with Bacterial DNA
D. S. Pisetsky, J. P. Grudier, and G. S. Gilkeson
Division of Rheumatology and Immunology, Duke University Medical Center, Durham,
NC 27705, USA
To investigate the role of DNA in the induction of anti-DNA antibodies in
systemic lupus erythematosus (SLE), the immune response of mice to bacterial
DNA was investigated. BALB/c mice immunized with E. coli (EC) single-stranded
DNA (ssDNA) in complexes with mBSA produced antibody levels much greater
than similar immunization with calf thymus (CT) DNA. The induced antibodies
contained a population specific for EC DNA as well as a population crossreactive
with CT as well as other DNAs. The induced antibodies bound moreover to a
variety of synthetic polynucleotides including poly dC, poly I, and poly di
without reactivity to poly dT or poly dU. In contrast, immunization with double-
stranded (ds) E. coli DNA elicited antibodies specific for ds E. coli DNA without
crossreactivity to mammalian DNA.