DOI Page / Citation link:
https://doi.org/10.11588/diglit.48120#0130
112 Molecular and Cell Biology of Autoantibodies and Autoimmunity
References
1. Krapf, F.E., Herrmann, M., Leitmann, W., Kalden, J. R.: Clin. Exp. Immunol,
(in press)
2. Herrmann, M., Leitmann, W., Krapf, E.F., Kalden, J.R.: J. Cell. Mol. Biol, (in
press)
Molecular Features of an RNA Recognition Motif Family of Proteins
J. D. Keene
Department of Microbiology and Immunology, Duke University Medical Center, Durham,
NC 27710, USA
A family of modular proteins has been recognized that can interact in a
specific manner with RNA and in some cases with other molecules. These pro-
teins contain a common primary sequence motif (RRM) of 80 amino acids that
is part of the RNA binding domain. Several members of the family are autoan-
tigenic in that they are components of small nuclear ribonucleoproteins of human
cells. The autoreactive members of the family include the 70 K, A, La, Ro and
B" proteins.
The RRM family of proteins can be divided into two classes, those that con-
tain a single copy of the RRM and those that contain two or more copies. Among
the members of the family that are known to be autoimmune, the La, 60 K Ro
and 70 K RNP proteins are within class I while the A and B" proteins are within
class II. Most members of the RRM family have some apparent role in RNA pro-
cessing although some members have also been implicated in transcription (La,
rho and Ro). It is possible that the entire family evolved from a common ancestral
gene within a functional network involving RNA processing.
The sites of recognition of RNA by several members of the RRM family have
been elucidated using a variety of methods including Northwestern blotting, im-
munoprecipitation of complexes and mobility shift assays. Together with nuclease
protection and in vitro synthesis of specific deletion mutants of the RNAs, bind-
ing sites have been assigned. These approaches have recently yielded definitive
assignments with the availability of recombinant vectors expressing the La, Ro,
70 K, A and B" proteins.
The RNA recognition motif is a candidate region for cross-reactivity among
the autoantigenic members of the family. However, since several members of the
family do not appear to react with autoimmune sera and because many autoan-
References
1. Krapf, F.E., Herrmann, M., Leitmann, W., Kalden, J. R.: Clin. Exp. Immunol,
(in press)
2. Herrmann, M., Leitmann, W., Krapf, E.F., Kalden, J.R.: J. Cell. Mol. Biol, (in
press)
Molecular Features of an RNA Recognition Motif Family of Proteins
J. D. Keene
Department of Microbiology and Immunology, Duke University Medical Center, Durham,
NC 27710, USA
A family of modular proteins has been recognized that can interact in a
specific manner with RNA and in some cases with other molecules. These pro-
teins contain a common primary sequence motif (RRM) of 80 amino acids that
is part of the RNA binding domain. Several members of the family are autoan-
tigenic in that they are components of small nuclear ribonucleoproteins of human
cells. The autoreactive members of the family include the 70 K, A, La, Ro and
B" proteins.
The RRM family of proteins can be divided into two classes, those that con-
tain a single copy of the RRM and those that contain two or more copies. Among
the members of the family that are known to be autoimmune, the La, 60 K Ro
and 70 K RNP proteins are within class I while the A and B" proteins are within
class II. Most members of the RRM family have some apparent role in RNA pro-
cessing although some members have also been implicated in transcription (La,
rho and Ro). It is possible that the entire family evolved from a common ancestral
gene within a functional network involving RNA processing.
The sites of recognition of RNA by several members of the RRM family have
been elucidated using a variety of methods including Northwestern blotting, im-
munoprecipitation of complexes and mobility shift assays. Together with nuclease
protection and in vitro synthesis of specific deletion mutants of the RNAs, bind-
ing sites have been assigned. These approaches have recently yielded definitive
assignments with the availability of recombinant vectors expressing the La, Ro,
70 K, A and B" proteins.
The RNA recognition motif is a candidate region for cross-reactivity among
the autoantigenic members of the family. However, since several members of the
family do not appear to react with autoimmune sera and because many autoan-