DOI Seite / Zitierlink:
https://doi.org/10.11588/diglit.48120#0051
Abstracts
33
firmed and extended the observations of others and shown that the HLA-DR4
phenotype is increased in North American Caucasian patients with MCTD. This
HLA-DR4 linked genetic susceptibility to Ul-68 Kd protein-positive MCTD ap-
pears to be distinct from the association of SLE with HLA-DR3, which was
found in our local SLE group and as has been reported in many other studies on
SLE. Also of interest from this study is the fact that we found no association of
Ul-68 Kd positive MCTD with any of the HLA antigens previously reported to
be associated with scleroderma, including HLA-A9, B8, DR3 and DR5.
We conclude that Ul-68 Kd protein-positive MCTD appears to be most
strongly associated with HLA-DR4 and/or haplotypes bearing the supratypic
marker HLA-DRw53. Ul-68 Kd protein-positive MCTD appears genetically
distinct from SLE which is associated with HLA-DR3 and HLA-DR2.
A Novel Trait of Naturally-Occurring Anti-DNA Antibodies:
Dissociation from Immune Complexes in Neutral 0.3 - 0.5 M NaCl
Y. Kanai1, T. Kubota2
' Department of Molecular Oncology, Institute of Medical Science, University of Tokyo,
Japan
2 First Department of Internal Medicine, Tokyo Medical and Dental University, Tokyo,
Japan
Monoclonal and polyclonal anti-DNA antibodies from autoimmune mice,
and experimentally-induced rabbit anti-nucleic acid polyclonal antibodies were
tested for stability of binding to nucleic acids in the presence of various concen-
trations of NaCl by an enzyme-linked immunosorbent assay (ELISA). Murine
monoclonal antibodies 200 (IgG2b) and 1A2 (IgG2a), which are known to react
specifically with double-stranded(ds) DNA, dissociated completely from their
complexes with DNA when washed with neutral 0.5 M NaCl solution. Another
monoclonal antibody (MoAb) (IgM, K), which is polyreactive with singlestrand-
ed(ss) DNA, cardiolipin, and trinitrophenyl hapten (TNP), was also dissociated
from its complexes with ssDNA, but not from its complexes with TNP, by
0.3-0.5 M NaCl. Similar differencies were observed in the stabilities of bindings
of serum antibodies from autommune mice to DNA and TNP. In contrast anti-
nucleic acid polyclonal antibodies induced in rabbits by immunization with po-
ly(I), poly(dT) or poly(ADP-ribose) were not significantly dissociated from their
immune complexes with relevant antigens or DNA by 0.5 M NaCl. The finding
33
firmed and extended the observations of others and shown that the HLA-DR4
phenotype is increased in North American Caucasian patients with MCTD. This
HLA-DR4 linked genetic susceptibility to Ul-68 Kd protein-positive MCTD ap-
pears to be distinct from the association of SLE with HLA-DR3, which was
found in our local SLE group and as has been reported in many other studies on
SLE. Also of interest from this study is the fact that we found no association of
Ul-68 Kd positive MCTD with any of the HLA antigens previously reported to
be associated with scleroderma, including HLA-A9, B8, DR3 and DR5.
We conclude that Ul-68 Kd protein-positive MCTD appears to be most
strongly associated with HLA-DR4 and/or haplotypes bearing the supratypic
marker HLA-DRw53. Ul-68 Kd protein-positive MCTD appears genetically
distinct from SLE which is associated with HLA-DR3 and HLA-DR2.
A Novel Trait of Naturally-Occurring Anti-DNA Antibodies:
Dissociation from Immune Complexes in Neutral 0.3 - 0.5 M NaCl
Y. Kanai1, T. Kubota2
' Department of Molecular Oncology, Institute of Medical Science, University of Tokyo,
Japan
2 First Department of Internal Medicine, Tokyo Medical and Dental University, Tokyo,
Japan
Monoclonal and polyclonal anti-DNA antibodies from autoimmune mice,
and experimentally-induced rabbit anti-nucleic acid polyclonal antibodies were
tested for stability of binding to nucleic acids in the presence of various concen-
trations of NaCl by an enzyme-linked immunosorbent assay (ELISA). Murine
monoclonal antibodies 200 (IgG2b) and 1A2 (IgG2a), which are known to react
specifically with double-stranded(ds) DNA, dissociated completely from their
complexes with DNA when washed with neutral 0.5 M NaCl solution. Another
monoclonal antibody (MoAb) (IgM, K), which is polyreactive with singlestrand-
ed(ss) DNA, cardiolipin, and trinitrophenyl hapten (TNP), was also dissociated
from its complexes with ssDNA, but not from its complexes with TNP, by
0.3-0.5 M NaCl. Similar differencies were observed in the stabilities of bindings
of serum antibodies from autommune mice to DNA and TNP. In contrast anti-
nucleic acid polyclonal antibodies induced in rabbits by immunization with po-
ly(I), poly(dT) or poly(ADP-ribose) were not significantly dissociated from their
immune complexes with relevant antigens or DNA by 0.5 M NaCl. The finding