DOI Seite / Zitierlink:
https://doi.org/10.11588/diglit.48120#0078
60
Molecular and Cell Biology of Autoantibodies and Autoimmunity
recently found to be a DNA polymerase-delta auxiliary protein. When the cDNA
for PCNA/cyclin was cloned and analyzed, both in the rat and human, it was
found that the protein consisted of 261 amino acids with a calculated molecular
weight of 28700. The amino acid sequences of the rat and man PCNA/cyclin were
the same, except the rat 7th amino acid He was Vai in man; the 33rd, Gly was Ser;
the 190th, Ser was Thr; and the 216th, Pro was Ser. The Southern blot analysis
of rat and human genomic DNA indicated that there is one copy of PCNA/cyclin
per haploid genome of the both species. The gene for PCNA/cyclin was assigned
to a long arm of human chromosome 2 at 2q33. The rat PCNA/cyclin cDNA pro-
be hybridized with homologous sequences in genomic DNAs from rice, soybean,
tobacco and red pepper and when the PCNA/cyclin-related molecular clone
isolated from rice DNA was used as a probe for RNA blot analysis, the probe
hybridized with a 1.2 kilobase transcript in RNA from rice root tips and shoots.
Immunohistochemically, in the proliferating cells, PCNA/cyclin first appeared in
the cytoplasm, migrated into nuclei and again in the cytoplasm in the region of
the Golgi complexes. With our study, at what exact stage of mitosis PCNA/cyclin
re-entered cytoplasm could not be defined, we presume it was during the prophase
since little or not PCNA/cyclin was associated with the metaphase chromosomes.
The strong conservation of the gene and the protein for PCNA/cyclin among
animal and plant kingdoms suggests the essential role of this protein in DNA
replication in eukaryotes.
Identification of Several Independent Autoreactive Epitopes of the
Human 68 kDa (U1) snRNP-Autoantigen
H.J. Netter1, H. H. Guldner1, C. Szostecki', H. J. Lakomek2, and H. Will1
'Max Planck Institut fur Biochemie, 8033 Martinsried, FRG
2Medizinische Klinik C, 4000 Dusseldorf, FRG
We have recently isolated a human cDNA coding for the human (U1) snRNP-
specific 68 kDa autoantigen. Using the purified recombinant p68 protein express-
ed in E. coli we established an ELISA-assay for p68 autoantibody detection
suitable for clinical routine [1]. Moreover, we recently identified three autoan-
tigenic domains of the p68 autoantigen [2].
To localize the autoreactive epitopes in more detail, to get an rough estimate
on how many independent epitopes are recognized by anti-p68 autoantibodies,
and whether patient specific patterns can be identified a detailed mapping of
Molecular and Cell Biology of Autoantibodies and Autoimmunity
recently found to be a DNA polymerase-delta auxiliary protein. When the cDNA
for PCNA/cyclin was cloned and analyzed, both in the rat and human, it was
found that the protein consisted of 261 amino acids with a calculated molecular
weight of 28700. The amino acid sequences of the rat and man PCNA/cyclin were
the same, except the rat 7th amino acid He was Vai in man; the 33rd, Gly was Ser;
the 190th, Ser was Thr; and the 216th, Pro was Ser. The Southern blot analysis
of rat and human genomic DNA indicated that there is one copy of PCNA/cyclin
per haploid genome of the both species. The gene for PCNA/cyclin was assigned
to a long arm of human chromosome 2 at 2q33. The rat PCNA/cyclin cDNA pro-
be hybridized with homologous sequences in genomic DNAs from rice, soybean,
tobacco and red pepper and when the PCNA/cyclin-related molecular clone
isolated from rice DNA was used as a probe for RNA blot analysis, the probe
hybridized with a 1.2 kilobase transcript in RNA from rice root tips and shoots.
Immunohistochemically, in the proliferating cells, PCNA/cyclin first appeared in
the cytoplasm, migrated into nuclei and again in the cytoplasm in the region of
the Golgi complexes. With our study, at what exact stage of mitosis PCNA/cyclin
re-entered cytoplasm could not be defined, we presume it was during the prophase
since little or not PCNA/cyclin was associated with the metaphase chromosomes.
The strong conservation of the gene and the protein for PCNA/cyclin among
animal and plant kingdoms suggests the essential role of this protein in DNA
replication in eukaryotes.
Identification of Several Independent Autoreactive Epitopes of the
Human 68 kDa (U1) snRNP-Autoantigen
H.J. Netter1, H. H. Guldner1, C. Szostecki', H. J. Lakomek2, and H. Will1
'Max Planck Institut fur Biochemie, 8033 Martinsried, FRG
2Medizinische Klinik C, 4000 Dusseldorf, FRG
We have recently isolated a human cDNA coding for the human (U1) snRNP-
specific 68 kDa autoantigen. Using the purified recombinant p68 protein express-
ed in E. coli we established an ELISA-assay for p68 autoantibody detection
suitable for clinical routine [1]. Moreover, we recently identified three autoan-
tigenic domains of the p68 autoantigen [2].
To localize the autoreactive epitopes in more detail, to get an rough estimate
on how many independent epitopes are recognized by anti-p68 autoantibodies,
and whether patient specific patterns can be identified a detailed mapping of