DOI Page / Citation link:
https://doi.org/10.11588/diglit.48120#0123
Abstracts
105
Autoantibodies to Nonhistone Chromosomal Proteins HMG-1 and
HMG-2 in Subsets of Juvenile Chronic Arthritis (JCA)
B. Wittemann1, F.A. Bautz1, H. Michels2, and H. Truckenbrodt2
1 Institute of Molekular Genetiks, University of Heidelberg, Im Neuenheimer Feld 230,
6900 Heidelberg, FRG
2 Rheumatic Children Hospital Garmisch-Partenkirchen, GehfeldstraBe 24,
8100 Garmisch-Partenkirchen, FRG
Antihistone antibodies (AHAs) in sera of patients suffering from juvenile
chronic arthritis (JCA) have been reported recently by several investigators [1, 2].
In our study of 100 patients with JCA admitted to the Rheumatic Children’s
Hospital in Garmisch-Partenkirchen we observed, in addition to antibodies
against various histones, autoantibodies against the nucleosomal proteins
HMG-1 and HMG-2. All sera tested were ANA positive by indirect immunofluo-
rescence on HEp-2 cells and negative when tested against double stranded DNA
by an indirect solid phase ELISA (Progen GmbH, Heidelberg). In the analysis re-
ported here we submitted purified HMG-1 and HMG-2 from calf-thymus [3] to
PAGE and subsequent western blots. The results presented in the table give a cor-
relation between clinical diagnosis and the presence of autoantibodies to
nucleosomal proteins HMG-1 and HMG-2 in JCA patients.
Table 1
* JCA with uveitis.
Clinical diagnosis
tested
(no)
positive
(no)
HMG-1
only
HMG-2
only
HMG-1 +
HMG-2
Polyarthritis
14 (2*)
4 (2*)
1
0
3
Oligoarthritis
86 (13*)
37 (3*)
10
7
20
Grouping the sera tested according to the clinical diagnosis (Table 1) reveals
a correlation between detectable amounts of antibodies to the chromosomal pro-
teins HMG-1 and HMG-2 and subsets of JCA. JCA patients with polyarthritis
react to 28%, whereas patients with oligoarthritis show a higher incident of 43%
binding to the high mobility group proteins 1 and 2.
105
Autoantibodies to Nonhistone Chromosomal Proteins HMG-1 and
HMG-2 in Subsets of Juvenile Chronic Arthritis (JCA)
B. Wittemann1, F.A. Bautz1, H. Michels2, and H. Truckenbrodt2
1 Institute of Molekular Genetiks, University of Heidelberg, Im Neuenheimer Feld 230,
6900 Heidelberg, FRG
2 Rheumatic Children Hospital Garmisch-Partenkirchen, GehfeldstraBe 24,
8100 Garmisch-Partenkirchen, FRG
Antihistone antibodies (AHAs) in sera of patients suffering from juvenile
chronic arthritis (JCA) have been reported recently by several investigators [1, 2].
In our study of 100 patients with JCA admitted to the Rheumatic Children’s
Hospital in Garmisch-Partenkirchen we observed, in addition to antibodies
against various histones, autoantibodies against the nucleosomal proteins
HMG-1 and HMG-2. All sera tested were ANA positive by indirect immunofluo-
rescence on HEp-2 cells and negative when tested against double stranded DNA
by an indirect solid phase ELISA (Progen GmbH, Heidelberg). In the analysis re-
ported here we submitted purified HMG-1 and HMG-2 from calf-thymus [3] to
PAGE and subsequent western blots. The results presented in the table give a cor-
relation between clinical diagnosis and the presence of autoantibodies to
nucleosomal proteins HMG-1 and HMG-2 in JCA patients.
Table 1
* JCA with uveitis.
Clinical diagnosis
tested
(no)
positive
(no)
HMG-1
only
HMG-2
only
HMG-1 +
HMG-2
Polyarthritis
14 (2*)
4 (2*)
1
0
3
Oligoarthritis
86 (13*)
37 (3*)
10
7
20
Grouping the sera tested according to the clinical diagnosis (Table 1) reveals
a correlation between detectable amounts of antibodies to the chromosomal pro-
teins HMG-1 and HMG-2 and subsets of JCA. JCA patients with polyarthritis
react to 28%, whereas patients with oligoarthritis show a higher incident of 43%
binding to the high mobility group proteins 1 and 2.