DOI Seite / Zitierlink:
https://doi.org/10.11588/diglit.48120#0124
106 Molecular and Cell Biology of Autoantibodies and Autoimmunity
References
1. Ostensen, M. et al. (1989): Identification of antihistone antibodies in subsets of juve-
nile chronic arthritis. Annals of the Rheumatic diseases 48, 114-117
2. Pauls, J. D., Fritzler, M. J. (1989): Juvenile Rheumatoid Arthritis antibodies bind
Histones Hl and H5. Arth. & Rheum. 32, No 1
3. Bustin, M. et al. (1982): Autoantibodies to Nucleosomal Proteins: Antibodies to
HMG-17 in Autoimmunie Diseases. Science 215, 1245-1247
A Human T Cell Clone Recognizing Small Nuclear Ribonucleoproteins
(UsnRNP)
G. Wolff-Vorbeck1, M. Schlesier1, W. Hackl2, R. Liihrmann2, and H.H. Peter1
‘Abt. Rheumatologie und klinische Immunologie, Medizinische Universitatsklinik,
Freiburg, FRG
2Institut fiir Molekularbiologie und Tumorforschung, Philipps Universitat, Marburg,
FRG
Autoantibodies to U1 snRNP are frequently found in patients with mixed
connective tissue disease (MCTD) and systemic lupus erythematosus (SLE). So
far it is not known, whether this autoantibody production is T cell-dependent.
From peripheral blood of a healthy donor we established a CD3+ T cell line,
composed of both CD4+ and CD8+ cells, which recognizes unfractionated
UsnRNP. By inhibition studies with monoclonal antibodies and testing of a panel
of HLA typed individuals, the cell line was shown to be HLA-DR4 restricted. The
cell line does not recognize control antigens such as PPD, collagen type II, pro-
teoglycan or mycobacterial 65 kD heat shock protein. From this T cell line an
UsnRNP-specific CD4+ T cell clone has been derived; its fine specificity is
under investigation using fractionated UsnRNP proteins. In addition, the autoan-
tigenspecific helper activity of the T cell clone for immunoglobulin production
by autologous B cells will be studied in vitro.
References
1. Ostensen, M. et al. (1989): Identification of antihistone antibodies in subsets of juve-
nile chronic arthritis. Annals of the Rheumatic diseases 48, 114-117
2. Pauls, J. D., Fritzler, M. J. (1989): Juvenile Rheumatoid Arthritis antibodies bind
Histones Hl and H5. Arth. & Rheum. 32, No 1
3. Bustin, M. et al. (1982): Autoantibodies to Nucleosomal Proteins: Antibodies to
HMG-17 in Autoimmunie Diseases. Science 215, 1245-1247
A Human T Cell Clone Recognizing Small Nuclear Ribonucleoproteins
(UsnRNP)
G. Wolff-Vorbeck1, M. Schlesier1, W. Hackl2, R. Liihrmann2, and H.H. Peter1
‘Abt. Rheumatologie und klinische Immunologie, Medizinische Universitatsklinik,
Freiburg, FRG
2Institut fiir Molekularbiologie und Tumorforschung, Philipps Universitat, Marburg,
FRG
Autoantibodies to U1 snRNP are frequently found in patients with mixed
connective tissue disease (MCTD) and systemic lupus erythematosus (SLE). So
far it is not known, whether this autoantibody production is T cell-dependent.
From peripheral blood of a healthy donor we established a CD3+ T cell line,
composed of both CD4+ and CD8+ cells, which recognizes unfractionated
UsnRNP. By inhibition studies with monoclonal antibodies and testing of a panel
of HLA typed individuals, the cell line was shown to be HLA-DR4 restricted. The
cell line does not recognize control antigens such as PPD, collagen type II, pro-
teoglycan or mycobacterial 65 kD heat shock protein. From this T cell line an
UsnRNP-specific CD4+ T cell clone has been derived; its fine specificity is
under investigation using fractionated UsnRNP proteins. In addition, the autoan-
tigenspecific helper activity of the T cell clone for immunoglobulin production
by autologous B cells will be studied in vitro.